This time lapse video shows how the 3D images of molecules in living cells are generated and rendered, based on a fluorescence microscopy technique developed at KTH. First, they disentangle a mitochondrial network and optically “slice” through an organelle, capturing multiple images that can be reconstructed in 3D. In this case, the rendering on the right reveals a small, round mitochondrion with a longer, larger one connect to each other.